Program-controlled quantitative sealing machine

1. Overview

The program-controlled quantitative sealing machine is a machine specially designed to meet the needs of modern packaging production. It is driven by intelligent circuits and has a dedicated internal heating roller. It is specially used for sealing 51-hole quantitative plates and 97-hole quantitative plates. It is used with microbial detection reagents to provide simple, fast and accurate quantitative detection of total coliform bacteria, fecal (heat-resistant) coliform bacteria, and Escherichia coli. The 51-hole quantitative plate and 97-hole quantitative plate are semi-automatic quantitative methods designed based on the maximum probable number (MPN) statistical model of traditional methods. They can quantitatively detect water sample results. This method can replace and is superior to traditional (membrane and multi-tube fermentation) methods for detecting microorganisms in water.

2. Scope of application:

It is used for rapid detection of total coliform bacteria, fecal (heat-resistant) coliform bacteria, and Escherichia coli in water samples, which can be carried in the field, emergency, and quantitative detection.

3. Implementation standards:

Comply with "GB 3838-2002 Surface Water Environmental Quality Standard"

Comply with "CJ/T 206-2005 Urban Water Supply Quality Standard"

Comply with "GB/T 5750.12-2023 Drinking Water Standard Test Method"

Comply with "CJ/T51-2018 Urban Sewage Water Quality Standard Test Method Heat-resistant Coliform Determination Enzyme Substrate Method"

Comply with "HJ 1001-2018 National Environmental Protection Standard Water Quality Determination of Total Coliform, Fecal Coliform and Escherichia Coli"

4. Product description:

The enzyme substrate method detection device consists of a program-controlled quantitative sealing machine, a 51 or 97-hole quantitative detection plate, a 100mL quantitative bottle, and an enzyme substrate method detection reagent. The detection range of water samples: drinking water, source water, bottled water, reclaimed water, secondary water supply, pipe network water, wastewater, food water, livestock water, medical water, etc.

Advantages and characteristics:

The enzyme substrate method is a standard method for detecting E. coli included in GB5750-2006. The enzyme substrate method is currently the most advanced method for detecting E. coli in water. It is gradually being recognized by domestic testing departments for its convenience, low false positives, and applicability to rapid testing of a large number of samples.

Compared with the multi-tube fermentation and membrane filter methods, the enzyme substrate method has greatly reduced detection steps and has low requirements for the experimental environment. The detection time can be reduced to 24 hours. It has a good application prospect in daily water sample monitoring and emergency monitoring, and can detect and prevent in time, minimizing the probability of major public safety accidents.

5. Advantages of the method:

1. No need to operate in a sterile room.

2. Manual operation time is less than 1 minute.

3. No need to prepare culture medium and sterilize a large number of glassware.

4. Qualitative and quantitative analysis can be completed in 24 hours, without verification test.

The MMO-MUG culture medium that complies with the fixed substrate technology enzyme substrate method of GB5750-2006 "Standard Test Method for Drinking Water" is equipped with a program-controlled quantitative sealing machine, and the test water sample is packaged with (51-well quantitative plate) or (97-well quantitative plate).

【Working Principle】Add water sample containing total coliform bacteria, and the target bacteria are cultured in Minimal Medium ONPG-MUG medium at 36℃±1℃. The specific enzyme β-galactosidase produced by total coliform bacteria can decompose the color source substrate ONPG in Minimal Medium ONPG-MUG medium, making the culture yellow; at the same time, Escherichia coli in the water sample produces specific β-glucuronidase to decompose the fluorescent substrate MUG in Minimal Medium ONPG-MUG medium, producing characteristic fluorescence. In the same principle, heat-resistant coliform bacteria (fecal coliform bacteria) will decompose the color source substrate ONPG in Minimal Medium ONPG-MUG medium when cultured at 44.5℃, making the medium yellow.

5. Product Features

High efficiency and energy saving: The layout of heating elements and temperature control algorithm are optimized, which not only improves work efficiency, but also reduces energy consumption.

Easy operation: Equipped with an intuitive and easy-to-understand touch screen interface, users can easily set working parameters; at the same time, it provides fault self-diagnosis function for easy daily maintenance.

Safe and reliable: with multiple safety measures such as overload protection and short circuit protection.

6. Technical parameters

Purpose: used for GB5750-2006 enzyme substrate method to detect total coliform group, Escherichia coli, fecal coliform group, etc. in water quality

Reliability: no leakage, no holes

Stability: can detect more than 50,000 samples, service life is more than 5 years

Convenience: with backspace key, quantitative disk counting, automatic energy saving function, one-button drainage function, large LCD display window, cleaning window, error prompt function

Convenience: No need for sterile room, 24h detection of total coliform bacteria, Escherichia coli, and fecal coliform bacteria in water

Weight: 14.2kg

Dimensions: 42cm long × 41cm wide × 33cm high

Preheating time: ≤3min

Noise: <48dba

Outer cover temperature: <40℃

Sealing speed: 10 seconds

Working voltage: AC 220V±10% 50HZ

Working efficiency: Sealing time of 51-hole and 97-hole quantitative detection plates ≤12 seconds/piece

Working environment temperature: -10℃~50℃

Detection range: 0-200MPN/100ml (water sample is not diluted) with 51-hole quantitative detection plate, 0-2419MPN/100ml (water sample is not diluted) with 97-hole quantitative detection plate

Product's Website：http://en.huoerd.com/mdwf/1941.html