Programmable Quantitative Sealer

Overview

The program-controlled quantitative sealer is designed to meet modern packaging production requirements. It is driven by an intelligent circuit and is equipped with a dedicated internal heating roller, specially used for sealing 51-well and 97-well quantitative trays. Used together with microbiological detection reagents, it provides a simple, rapid and accurate quantitative detection solution for total coliforms, fecal (thermotolerant) coliforms and Escherichia coli. The 51-well and 97-well quantitative trays are semi-automatic quantitative methods designed based on the traditional most probable number (MPN) statistical model, and can quantitatively detect water sample results. This method can replace and outperform traditional methods (membrane filtration and multiple-tube fermentation) for detecting microorganisms in water.

Scope of Application

It is used for the rapid detection of total coliforms, fecal (thermotolerant) coliforms and Escherichia coli in water samples; it can be carried to the field for emergency and quantitative detection.

Executive Standards

• Complies with GB 3838-2002 Environmental Quality Standards for Surface Water
• Complies with CJ/T 206-2005 Water Quality Standards for Urban Water Supply
• Complies with GB/T 5750.12-2023 Standard Examination Methods for Drinking Water
• Complies with CJ/T 51-2018 Standard Examination Method for Urban Sewage Quality - Determination of Thermotolerant Coliforms by Enzyme Substrate Method
• Complies with HJ 1001-2018 National Environmental Protection Standard - Determination of Total Coliforms, Fecal Coliforms and Escherichia coli in Water Quality

Product Description

The enzyme substrate method detection device consists of four parts: the program-controlled quantitative sealer, the 51-well or 97-well quantitative detection tray, the 100 mL quantitative bottle, and the enzyme substrate method detection reagent. The water sample range covers drinking water, source water, bottled water, reclaimed water, secondary water supply, pipe network water, wastewater, food water, livestock water, medical water, etc.

The enzyme substrate method is a standard method for coliform detection included in GB 5750-2006. It is currently the most advanced method for detecting coliforms in water. With advantages such as convenience, rapidity, low false positive rate and suitability for rapid detection of large numbers of samples, it is gradually being recognized by domestic testing departments. Compared with the multiple-tube fermentation and membrane filtration methods, the enzyme substrate method greatly reduces the detection steps, has low requirements on the experimental environment, and can reduce the detection time to 24 hours. It has good application prospects in routine water sample monitoring and emergency monitoring, and can detect and prevent in time, minimizing the probability of major public safety accidents. Taking the determination of total coliforms in GB 5750-2006 as an example, the three methods are compared in the table below.

Method Advantages

• No need to operate in a sterile room.
• Manual operation time is less than 1 minute.
• No need for medium preparation or sterilization of large amounts of glassware.
• Qualitative and quantitative analysis can be completed in 24 hours, without verification tests.
• In accordance with GB 5750-2006 Standard Examination Methods for Drinking Water, the MMO-MUG medium of the fixed substrate technology enzyme substrate method is used together with the program-controlled quantitative sealer, and the water sample is sealed with a (51-well quantitative tray) or (97-well quantitative tray).

Working Principle

Add a water sample containing total coliform bacteria; the target bacteria are cultured at 36℃±1℃ in Minimal Medium ONPG-MUG. The specific biological enzyme β-galactosidase produced by total coliform bacteria can decompose the chromogenic substrate ONPG in the Minimal Medium ONPG-MUG, turning the culture yellow; at the same time, Escherichia coli in the water sample produces a specific β-glucuronidase that decomposes the fluorescent substrate MUG in the Minimal Medium ONPG-MUG, producing characteristic fluorescence. By the same principle, thermotolerant coliforms (fecal coliforms) decompose the chromogenic substrate ONPG in the Minimal Medium ONPG-MUG when cultured at 44.5℃, turning the medium yellow.

Product Features

• High efficiency and energy saving: the heating element layout and temperature control algorithm are optimized, which not only improves work efficiency but also reduces energy consumption.
• Simple operation: equipped with an intuitive touch-screen interface, users can easily set working parameters; a fault self-diagnosis function is also provided for routine maintenance.
• Safe and reliable: equipped with multiple safety measures such as overload protection and short-circuit protection.

Technical Specifications