Plant root image analyzer

1. Instrument use:

The root analysis system is used for professional root analysis after root washing, and can also be used for root phenotype analysis of plants cultured in root boxes. It can analyze root length, diameter, area, volume, root tip count, etc. It is powerful and easy to operate. The software can analyze the morphological analysis of plant roots and the overall structural distribution of the root system, etc. It is widely used in root morphology and structure research.

2. Instrument principle:

The root analysis system uses a high-quality graphic scanner to obtain high-resolution color or black-and-white images of plant roots. The scanner is equipped with a special dual-light source lighting system under the scanning panel and in the upper cover, and a dual-light source calibration area is reserved on the scanning panel. In addition, it is equipped with special, high-transparency root placement trays of different sizes. During scanning, the light source under the scanning panel and the light source in the upper cover simultaneously scan the root samples in the high-transparency root tray, which can avoid the influence of shadows and unevenness that are easy to occur during root scanning, and effectively ensure the quality of the acquired image.

This soft root analysis software can read images in TIFF and JPEG standard formats. For the acquired images, the high-quality root images obtained by scanning are analyzed using software that is decrypted by inserting a dongle. The basic morphological parameters such as root length, diameter, area, volume, and root tip of the cross-overlapping parts are measured and calculated using non-statistical methods. This meets the researchers' research on different categories and levels of plant roots.

3. Technical indicators:

1. Equipped with an optical resolution of 4800×9600 and an A4-lengthened dual-light source color scanner. The root reflection draft format is 355.6mm×215.9mm, the through-scan format is 320.0mm×203.2mm, and the minimum pixel size is 0.005mm×0.0026 mm.

2. Analytical measurements:

(1) Total root length;

(2) Branching frequency;

(3) Average root diameter;

(4) Median root diameter;

(5) Maximum diameter;

(6) Total root area;

(7) Total projected area;

(8) Total root volume;

(9) Root tip count;

(10) Fork count;

(11) Overlap count;

(12) Root diameter grade distribution parameters;

(13) Customizable segmented diameters at unequal intervals, automatically measuring the length, projected area, surface area, volume, etc. of each diameter segment, and its distribution parameters.

(14) Root color analysis can be performed, and the diameter, length, projected area, surface area, and volume of roots of different colors can be output.

(15) Topological analysis of the root system can be performed, automatically determining the number of root connections, relationship angles, etc., and the length, area, volume, etc. of any root segment can be automatically analyzed separately, and the corresponding parameters of any diameter segment of the marked root system can be displayed separately (customizable at unequal intervals).

(16) It can perform root bifurcation, pruning, merging, and connection corrections, and the correction operation can be rolled back to quickly obtain 100% correct results.

(17) It can automatically measure the fractal dimension of the root system using the box dimension method. It can analyze the number of nodules to objectively determine the contribution of rhizobia.

(18) Fully automatic root system analysis of large quantities, batch saving, the overall data can be summarized and saved in a table, and detailed data of each root system can also be saved.

(19) Extract analysis characteristics of ground angle analysis and horizontal angle analysis.

(20) Each analysis image, distribution map, and result data can be saved and exported to Excel, and analysis mark map can be exported.

(21) The instrument has cloud platform support, and the analysis data can be saved to the cloud for viewing anytime and anywhere

(22) Built-in Chinese and English bilingual display, one-click switching, seamless docking

4. Image capture system parameters

Scanning element: 6-line alternating microlens CCD

Maximum format: A4

Interface type: USB2.0

Optical resolution (dpi): 4800x9600dpi

Maximum resolution 12800×12800dpi

Minimum pixel size ≥0.005mm×0.0026 mm

Scanning light source white cold cathode fluorescent lamp CCFL, color bit 48 bits

Scanning range 216×297mm

Scanning speed reflective draft, A4, 300dpi: monochrome 11 seconds, color 14 seconds

Film scanning, 35mm, 2400dpi: positive: 47 seconds, negative: 44 seconds

5. Others

1. This product requires the use of a computer, recommended options: brand computer (Core i5 9th generation or above CPU / 16G memory / 21.5” color display/wireless network card, more than 4 USB2.0 ports, running environment Windows 10 full professional or ultimate edition).

2. Optional A3 format dual light source color scanner. Reflective draft scanning format 305mm × 431.8mm, root system through scanning format 304.8mm × 406.4 mm.

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